首页> 外国专利> METHOD FOR DIAGNOSING SCHIZOPHRENIA, SET OF TWO PRIMERS FOR REALIZING THE METHOD AND KIT COMPRISING THE SET OF PRIMERS

METHOD FOR DIAGNOSING SCHIZOPHRENIA, SET OF TWO PRIMERS FOR REALIZING THE METHOD AND KIT COMPRISING THE SET OF PRIMERS

机译:诊断精神分裂症的方法,用于实现该方法的两个主体的集合以及包括主体的集合的工具包

摘要

1. A method for diagnosing schizophrenia, characterized in that said method being based on the detection in vitro of the presence of the allele Ep of the microsatellite DNA HUMTHO1 in the gene TH. 2. The method of claim 2, characterized in that the detection of the by allele Ep is realized by sequenation of DNA and/or by separation of DNA fragments in gel and/or by separation of DNA fragments in gel in non-denaturing conditions. 3. The method of claim 2, characterized in that the allele Ep is detected by separation of DNA fragments in gel. 4. The method of any claim 1-3, characterized in that using DNA extracted from mononuclear cells. 5. The method of any claim 1-4, characterized in that prior to detection of the allele Ep DNA is amplified. 6. The method of claim 5, characterized in that DNA is amplified, comprising the first intron of the TH full length or part of said intron. 7. The method of claim 6, characterized in that DNA is amplified which is a fragment of less than 300 pb, comprising microsatellite HUMTHO1 and flanking sequences. 8. The method of claim 6, characterized in that DNA is amplified which is a fragment of less than 200 pb, preferably less 160 pb. 9. A set of two primers for realizing the method of claim 8, having the following structure (pair A): 5' GGC AAA TAG GGG GCA AAA 3' (sense sequence) 5' TTA TCC AGC CTG GCC CAC 3' (anti-sense sequence) 10. A set of two primers for realizing the method of claim 8, having the following structure (pair B): 5' GGC AAA TAG GGG GCA AAA 3' (sense sequence) 5' GGC TTC CGA GTG CAG GTC 3' ( anti-sense sequence) 11. A set of two primers for realizing the method of claim 8, having the following structure (pair C): 5' GTT CCT CCC TTA TTT CCC 3' (sense sequence) 5' AGG GAA CAC AGA CTC CAT (anti-sense sequence) 12. A kit for detecting of the allele Ep of the microsatellite DNA HUMTHO1, comprising a set of primers of any claim 9-11. 13. A kit of claim 12 for diagnosing schzophrenia.
机译:1.一种诊断精神分裂症的方法,其特征在于所述方法基于体外检测基因TH中微卫星DNA HUMTHO1的等位基因Ep的存在。 2.根据权利要求2所述的方法,其特征在于,通过等位基因Ep的检测是通过DNA的分离和/或通过凝胶中DNA片段的分离和/或通过在非变性条件下凝胶中DNA片段的分离来实现的。 3.权利要求2的方法,其特征在于通过在凝胶中分离DNA片段来检测等位基因Ep。 4.权利要求1-3中任一项的方法,其特征在于使用从单核细胞提取的DNA。 5.根据权利要求1-4中任一项所述的方法,其特征在于,在等位基因Ep DNA检测之前,将其扩增。 6.权利要求5的方法,其特征在于DNA被扩增,其包含TH全长的第一内含子或所述内含子的一部分。 7.根据权利要求6所述的方法,其特征在于,扩增的DNA是小于300pb的片段,其包含微卫星HUMTHO1和侧翼序列。 8.权利要求6的方法,其特征在于扩增的DNA是小于200pb,优选小于160pb的片段。 9.用于实现权利要求8的方法的一组两个引物,其具有以下结构(对A):5'GGC AAA TAG GGG GCA AAA 3'(有义序列)5'TTA TCC AGC CTG GCC CAC 3'(反10.用于实现权利要求8的方法的一组两个引物,其具有以下结构(B对):5'GGC AAA TAG GGG GCA AAA 3'(正义序列)5'GGC TTC CGA GTG CAG GTC 11.用于实现权利要求8的方法的两个引物的集合,其具有以下结构(对C):5'GTT CCT CCC TTA TTT CCC 3'(有义序列)5'AGG GAA CAC AGA CTC CAT(反义序列)12.用于检测微卫星DNA HUMTHO1的等位基因Ep的试剂盒,其包含权利要求9-11中任一项的引物组。 13.根据权利要求12所述的试剂盒,用于诊断精神分裂症。

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号