BIFUNCTIONAL UROCINASE-VARIANTS HAVING IMPROVED FIBRINOLYTIC PROPERTIES AND THROMBIN-INHIBITING ACTIVITY

摘要

Bifunctional space kinase variants with improved fibrinolytic lytic needle degeneration and throbin inhibitory activity, plasmids for use in the preparation of these polypeptides, and single-bifunctional space kinase variants containing the active ingredient are added to the trypticolytic enzymes. The bifunctional space kinase variants of the present invention correspond to the general formula (I), wherein M4 is as defined in FIG. Figure 47A shows the amino acid sequence of the non-glycosylated prebase kinase 47Ser and 411Leβ; X1 is a bond between M4 and Y1, or a Ser-Pro-Pro-Pro-Ser-Pro-Pro-Gly-Gly-Ser-Pro-Pro-Ser-Pro-Pro-Ser-Pro-Pro-Gly-Gly-PhevagySer -Pro-Pro-Ser-Pro-Pro-Pro-Ser-Pro-Pro-Gly-Gly-Phe-Glycic peptide; or a peptide sequence of general formula (II) wherein X2 is Pro or Leu; X 3 represents Val or Prő; X 4 is Lys, Val, Arg, Gly or Gly; X 5 is Ala, Val, Gly, Leû or Ile; X6 is Phe, Trp, Tyr or Val, and X7 is Gly or a direct bond between X6 and Y1 -; and Y 1 is a Y 2 -Arg-Pr-Y 3 -Gly-Gly-Gly-Gly-Asn-Gly-Asp-Phe-Gly-Gly-Ile-Pr-Gly-Gly-Tyr-Leyl-Y 4; or aY2-Arg-Pr-Phe-Leu-Le-Arg-Asn-Pr-Asn-Asp-Lys-Tyr-Gly-Pr-Phe-Trp-Gly-Asp-Gly-Gly-Lys-Asn-Gly ; or a peptide of the general formula Y 2 -Arg-Pr-Ser-Ser-Gly-Phe-Gly-Gly-Phe-Gly-Ile-Asp-Gly-Gly-Gly-Gly-Lys, wherein Y 2 is Pr or Val; Y3 is Leu or a direct bond between Pro and Gly, and Y4 is Gln or hydroxy tube. ŕ