isolated polypeptide comprising an immunogenic portion of a M vaccine antigen, use in the treatment and diagnosis of mycobacterial infections

摘要

The isolated polypeptide comprises an immunogenic portion of M. vaccae antigen. The antigen includes a sequence selected from: a) sequences recites in SEQ ID 1-4, 9-16, 18-21, 23, 25, 26, 28, 29, 44, 45, 47, 52-55, 63, 64, 70, 75, 89, 94, 98, 100-105, 109, 110, 112, 121, 124, 125, 134, 135, 140 and 141 depicted in the specification and b) sequences having at least 99% probability of being the same as sequence recited in SEQ ID 1-4, 9-16, 18-21, 23, 25, 26, 28, 29, 44, 45, 47, 52-55, 63, 64, 70, 75, 89, 94, 98, 100-105, 109, 110, 112, 121, 124, 125, 134, 135, 140 and 141 depicted in the specification as measured by computer algorithm BLASTP or the antigen comprises an amino acid sequence encoded by a DNA molecule selected from: c) sequences recited in SEQ ID: 40-42, 46, 48-51, 74, 88, 93, 97, 99, 106-108, 111, 120, 122, 123, 132, 133 and 136-138 depicted in the specification; d) complements of SEQ ID: 40-42, 46, 48-51, 74, 88, 93, 97, 99, 106-108, 111, 120, 122, 123, 132, 133 and 136-138 depicted in the specification and e) sequences having at least 99% probability of being the same as sequence of c) and d) as measured by a computer algorithm FASTA. SEQ ID 1 is Ala, Pro, Val, Gly, Pro, Gly, Xaa, Ala, Ala, Tyr, Val, Gln, Gln, Val, Pro, Asp, Gly, Pro, Gly, Ser, Val, Gln, Gly, Met, Ala. An isolated DNA molecule having a nucleotide sequence encoding the above-defined polypeptide, the expression vector and the host cell thereof are disclosed. A pharmaceutical composition and vaccine thereof useful for inducing protective immune in a patient.