Analysis method and reagent kit for the quantitative in vitro determination in biological specimens of the activity of proteins that cause multidrug resistance in tumors.

摘要

HERE ARE describes a method DIAGNOSTIC TEST KITS FOR DETERMINING QUANTITATIVE REAGENT IN VITRO ACTIVITY IN BIOLOGICAL SPECIMENS CAUSING PROTEIN MDR multidrug resistance in tumors. THE METHOD OF THE INVENTION IS BASED ON THE MEASUREMENT OF SPEED COMPOUND accumulation in cells of the specimen (advantageously by fluorescence measurement); This compound is not a good protein substrate MDR PRESENT IN THE SAMPLE NO pervious CELL AND BEING FORMED IN CELLS, by generally present in cells ENZYMES, FROM A compound which is a good substrate of said protein MDR pervious CELL AND IN VITRO the specimen is added. Based on the method of the invention it is possible to calculate a factor value MDR activity (MAF) IS IN THE RANGE OF RELEVANT DIAGNOSIS, CHARACTERISTIC OF ACTIVITY MDR, THAT IS, THE LEVEL OF RESISTANCE multidrug OF SPECIMEN .