IMPROVED METHOD FOR PURIFICATION OF RECOMBINANT COPPER/ZINC (CU-ZN) SUPEROXIDE DISMUTASE FROM BACTERIA OR EUCARYOTIC CELLS

摘要

The subject invention provides a method for recovering a solution containingpurified, enzymatically active Cu-Zn superoxidedismutase or a polypeptide analog thereof having substantially the same aminoacid sequence as, and the biological activityof, naturally-occurring Cu-Zn superoxide dismutase from a composition whichcomprises cells containing Cu-Zn superoxide dismutaseor a polypeptide analog thereof. The invention also provides a method ofincreasing the yield of recovered solutions havingan increased concentration of b isoform of an enzymatically-active polypeptideanalog of Cu-Zn superoxide dismutase froma composition which comprises cells containing a, b and c isoforms of thepolypeptide analog.