XANTHAN-ESTER AND ACRIDAN SUBSTRATES FOR HORSERADISH PEROXIDASE

摘要

Xanthan-esters and acridans are substrates for horseradish peroxidase. Thesestable, enzymatically cleavable chemiluminescent esters are substrates forhorseradish peroxidase which, together with peroxide is among the extensivelyused enzyme in enzyme-linked detection methods, including immunoassays,oligonucleotide detection and nucleic acid hybridization. The novel compoundsare used, together with peroxide, alkali and the peroxidase, to indicate thepresence and/or concentration of target compounds. The assays may be enhancedby the use of polymeric quaternary onium enhancement compounds or similarcompounds selected to enhance the chemiluminescence emitted.