GENETIC AND EPIGENETIC MANIPULATION OF ABC TRANSPORTERS AND ECTO-PHOSPHATASES FOR THE CONFERENCE OF DRUG RESISTANCE AND FOR THE LOSS OF DRUG RESISTANCE IN BIOLOGICAL SYSTEMS AND METHODS FOR THE DETECTION OF ECTO-PHOSPHATASE INHIBITORS

摘要

The present invention relates to methods for modulating the resistance ofcells to foreign compounds, i.e. drugs, antibiotics, etc by altering the ATPgradient across biological membranes. The altering of the ATP gradient acrossbiological membranes is achieved through the manipulation of ecto-phosphataseactivity and ABC transporter molecule activity which may be useful to conferherbicide resistance to plants, confer antibiotic resistance to bacteria,confer drug resistance to yeast cells, or to reduce resistance in cells tofacilitate chemotherapeutic treatments, and to reduce resistance in bacteriaand yeast. The present invention is also directed to the methods foridentifying ecto-phosphatase inhibitors and uses thereof.