Process for causing plant cells and plants to code lysine or methionine or tryptophan or threonine at mrna codons other than those normally specifying these particular amino acids during protein synthesis

摘要

The present invention relates to a method for causing plant cells or plant tissue or plants to code lysine or methionine or tryptophan or threonine at mRNA codons other than those normally specifying those particular amino acids during protein synthesis, which comprises: i) preparing a DNA to express a tRNA which is charged with lysine and which binds to mRNA codons other than AAA or AAG during protein synthesis; or a DNA to express a tRNA which is charged with methionine and which binds to mRNA codons other than AUG; or a DNA to express a tRNA which is charged with tryptophan and which binds to mRNA codons other than UGG; or a DNA to express a tRNA which is charged with threonine and which binds to codons other than ACU, ACC, ACA, ACG; ii) introducing one or more of the said DNAs into plant protoplasts, plant cells or plants.