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METHOD FOR HIGH SPECIFIC BIOPRODUCTIVITY OF ALPHA, OMEGA-ALKANE DICARBOXYLIC ACIDS USING CANDIDA TROPICALIS
METHOD FOR HIGH SPECIFIC BIOPRODUCTIVITY OF ALPHA, OMEGA-ALKANE DICARBOXYLIC ACIDS USING CANDIDA TROPICALIS
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机译:利用热带念珠菌提高阿尔法,欧米茄-烷烃二羧酸的生物生产力的方法
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摘要
This invention provides a low-cost method of producing a,w-alkanedicarboxylic acids. Particular bioconversion conditions result in highly efficient conversion of fatty acid, fatty acid ester, or alkane substrates to diacids. Candida tropicalis AR40 or similar yeast strains are grown in a medium containing a carbon source and a nitrogen source at a temperature of 31 °C to 38 °C, while additional carbon source is continuously added, until maximum cell growth is attained. Within 0-3 hours of this point, substrate is added to the culture to initiate conversion. An a,w-alkanedicarboxylic acid made according to this method is also provided.
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