CD20-SPECIFIC REDIRECTED T CELLS AND THEIR USE IN CELLULAR IMMUNOTHERAPY OF CD20+ MALIGNANCIES

摘要

Genetically engineered, CD20-specific redirected T cells expressing a cell surface protein having an extracellular domain comprising a receptor which is specific for CD20, an intracellular signaling domain, and a transmembrane domain. Use of such cells for cellular immunotherapy of CD20+ malignancies and for abrogating any untoward B cell function. In one embodiment, the cell surface protein is a single chain FvFc: zeta receptor where Fv designates the VH and VL chains of a single chain monoclonal antibody to CD20 linked by peptide, Fc represents a hinge-CH2-CH3 region of a human IgG1, and zeta represents the intracellular signaling domain of the zeta chain of human CD3. A method of making a redirected T cell expressing a chimeric T cell receptor by electroporation using naked DNA encoding the receptor.