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CHROMATOGRAPHIC METHOD FOR MUTATION DETECTION USING MUTATION SITE SPECIFICALLY ACTING ENZYMES
CHROMATOGRAPHIC METHOD FOR MUTATION DETECTION USING MUTATION SITE SPECIFICALLY ACTING ENZYMES
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机译:突变位点特异作用酶的层析检测方法
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摘要
A method for analyzing a sample of double stranded DNA to determine the presence of a mutation therein comprises contacting the sample with a mutation site binding reagent, and chromatographically separating and detecting the product. The chromatographic separation can be performed using Matched Ion Polynucleotide Chromatography, size exclusion chromatography, ion exchange chromatography, or reverse phase chromatography. The mutation site binding reagent can be an enzyme or a non-proteinaceous chemical reagent. In one embodiment, a mutation site binding reagent binds to the site of mutation and alters the chromatographic retention time. In another embodiment, a mutation site binding reagent cleaves at the site of mutation, resulting in an increase in the number of fragments.
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