Cloning of human cyclin B2 gene and production and use of anti-human cyclin B2 antibody

摘要

The present invention relates to a method for preparing a human cyclin B2 gene and an antibody, and using the same to diagnose and treat the proliferation, division and cancer differentiation of human tissues or cells. (1) isolation and identification by cloning of human cyclin B2 gene, which is known as a subunit of regulating mitosis promoting factor (M-phase / maturation promoting factor (MPF) (3) Purification and extraction of human cyclin B2 protein produced by expression vector, (4) Production of polyclonal and monoclonal anti-human cyclin B2 antibody And (5) an assay for cancer testing using an anti-human cyclin B2 antibody. In addition, the human cyclin B2 gene of the present invention can be used as a valuable material in studying the cell cycle and proliferation of human cells, as well as being capable of promoting cell proliferation and cancer differentiation in human normal tissue cells, benign cancer tissue cells, It is used specifically as a cancer diagnosis reagent which can distinguish between benign and malignant cancer.