FIXED QUANTITY AND TYPE OF HPV USING CHAIN REACTION OF POLYMERASE AND UNUSUAL HYBRIDIZATION OF DNA/RNA SEQUENCE

摘要

PURPOSE: A fixed quantity and a type of HPV are provided to improve the limitation, a defect and inefficiency of general detecting methods. CONSTITUTION: A fixed quantity and a type of HPV(human papilloma virus) are confirmed by the following sequences. A standard DNA competitively reacting to HPV Genome in a sample of a patient in the reaction of HPV PCR(polymerase chain-reaction) while not interfering to HPV typing is developed. The amount of HPV Genome in the DNA of the patient is confirmed after performing PCR reaction by mixing the DNA extracted from the sample with the HPV standard DNA previously fixed in the quantity. Herein, a biotin is attached on a primer for HPV typing. DNA probes available for unusual reaction with respective HPV types are developed. The respective DNA probes are fixed on the surface of a membrane. The PCR result is spread on the membrane, hybridized and cleansed. An enzyme reagent attachable by discriminating a reaction biotin is spread on the cleansed membrane, attached thereon and cleansed. A coloring reaction is performed by spreading a coloring reactive base material of the enzyme reagent on the cleansed membrane for the discrimination.