METHOD FOR PRODUCING Mature Bone Bone Inducer

摘要

The expression vector of the expression vector and the processing enzyme of bone inductive factor precursor was introduced into the animal cell line, by culturing the resulting cell line producing mature bone inductive factor, comprising the step of separating the mature bone inducer from the culture, or , or bone induction factor was added to a solution of the processing enzyme in the precursor solution produce a mixture, and a process for producing mature bone induction factor by operating the processing enzyme, the bone inductive factor, comprising the step of incubating the thus obtained mixed solution disclosed do. Suitable methods are, who established (established) the expression vector of the expression vector, and secreted-purin variants of MP52 precursor introduced into the animal cell lines, and culturing the resulting cell line producing mature bone inductive factors and to separate them from the culture and a step.