VARIABLE REGION OF MURINE ANTIBODY AGAINST PRE-S1 SURFACE ANTIGEN OF HEPATITIS -B VIRUS , ITS C-DNA AND DNA SEQUENCE THEREOF

摘要

PURPOSE: Provided is cDNA gene coding rat's monoclonal antibody KR359 which binds with 21-28 amino acids of antigen pre-S1 epitope of hepatitis B virus in order to use the gene to bind with human antibody genes and consequently develop human antibodies with fewer side effects. CONSTITUTION: cDNA gene coding rat's monoclonal antibody KR359 is obtained by the following steps of: i) extracting RNA from hybridoma cell line (KCTC 0284 BP) and synthesizing cDNA for light and heavy chains; ii) implementing PCR with cDNA as a template and a primer of sequence ID. No. 1 of 5'-GCAGTCGACT GAGGCACCTC CAGATGTTAA C-3' for a light chain and a primer of sequence ID. No. 2 of 5'-CGGTCGACAG GGATCCAGAG TTCCAGGTCA C-3' for a heavy chain; iii) and obtaining 420bp cDNA of sequence ID. No. 8 described as in the description as a light chain and 500bp cDNA of sequence ID. No. 9 described as in the description as a heavy chain. Wherein, cDNA is also used in constructing plasmid vectors pKR359H and pKR359K.