BACTERIAL STRAIN BACILLUS CEREUS B 3B FOR OXIDATION OF PETROLEUM HYDROCARBONS AND DERIVATIVES

摘要

FIELD: biotechnological methods for oil pollution removal. SUBSTANCE: bacterial strain isolated from soil polluted by petroleum derivatives is cultured for 72 h in liquid nutrient medium containing mineral salts, soya been flour, and molasses at 28 C under aerobic conditions. Deep culture of microorganism (10%) is placed into sterile peat with mineral additives and additionally grown for 120 h at 3 C. Destructive activity of preparation containing thus grown strain with regard to petroleum hydrocarbons and derivatives at working concentration of bacteria 0.1% ranges between 91.5 and 94% within a wide pH range: from 3.0 to 8.5. EFFECT: enhanced oil destructive activity. 2 tbl, 2 ex