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Novel methods and primers for genetic analysis of biological material by polymerase chain reaction using specific primer pairs useful in, e.g. forensic medicine

机译:通过聚合酶链式反应,使用特定的引物对对生物材料进行遗传分析的新方法和引物,可用于例如法医学

摘要

Determining the origin of biological material by PCR using specific primers pairs, which are exclusively complementary to the respective animal or plant DNA is new. Detecting the origin of biological material comprises: (a) isolating the DNA from the investigation material; (b) use of - 1 specific primer pair where each pair comprises primers, which are exclusively complementary to the detected DNA; (c) duplicating the animal, or preferably plant DNA by PCR; and (d) detecting of the specific PCR-products by means of electrophoresis.
机译:通过使用特异性与各自动物或植物DNA互补的特异性引物对通过PCR确定生物材料的来源是新的。检测生物材料的来源包括:(a)从研究材料中分离DNA; (b)使用1对特异性引物,其中每对包含引物,这些引物与检测到的DNA完全互补; (c)通过PCR复制动物或优选地复制植物DNA; (d)通过电泳检测特定的PCR产物。

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