reagent for the enzymatic determination of bikarbonatgehalts in serum

摘要

PCT No. PCT/AU94/00392 Sec. 371 Date Nov. 29, 1995 Sec. 102(e) Date Nov. 29, 1995 PCT Filed Jul. 13, 1994 PCT Pub. No. WO95/07999 PCT Pub. Date Mar. 23, 1995The invention provides a reagent for enzymatic determination of serum bicarbonate levels in a patient wherein the degree of oxidation of a coenzyme is measured and said reagent is stabilized against oxidation by a coenzyme reduction system comprising an enzyme and substrate pair selected to enable continuous regeneration of said coenzyme throughout storage of said reagent. The invention also provides an improvement in an enzymatic method of determination of the concentration of serum bicarbonate in a sample body fluid wherein the degree of oxidation of a coenzyme is measured, the improvement comprising stabilizing a reagent which comprises the coenzyme against oxidation by a coenzyme reduction system comprising an enzyme and substrate pair selected so as to enable continuous regeneration of the coenzyme throughout storage of the reagent.