Column specific parameters for retention time locking in chromatography

摘要

The present invention provides an improved method for calculating and adjusting the column head pressure of a GC instrument based on the column's plate capacity, N.sub.c, and film thickness, d.sub. s, measured ideally during the manufacturing process and identified with the column such that software associated with the GC instrument can calculate a new column head pressure based on the relationship between the plate capacity and film thickness of the existing column to the plate capacity and film thickness of the new or shortened column. The column head pressure of the GC is adjusted to the new column head pressure to provide a reduction in the column-to-column non-reproducibility error in retention times of all peaks.