Phosphatase activated crosslinking, conjugating and reducing agents; methods of using such agents; and reagents comprising phosphatase activated crosslinking and conjugating agents

摘要

The present invention provides crosslinking, conjugating and reducing agents which are functional with at least one phosphorothioate monoester group (--SPO.sub.3.sup.-2). Crosslinking and conjugation methods as well as solid phase reagents and conjugates which are useful in immunoassays are also provided.PPCrosslinking and conjugating agents of the invention generally comprise a compound corresponding to the formula (I), shown below, wherein n at least 1 and Q is a straight or branched monomer, polymer or oligomer having an average molecular weight between about 200 and about 1,000,000. Additionally, when n is 1, Q comprises at least 1 additional reactive functionality. PP Q--(S--PO. sub.3.sup.-2).sub.n (I)PPThe reducing agents that are provided conform to a compound of the formula (Y) , shown below, wherein (A) and (Z) can be independently selected from C. sub.1 -C. sub.5 alkyl and CONH(CH.sub.2).sub.p wherein p is an integer between 1 and 5. ##STR1##