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Method of defining cell types by probing comprehensive expression libraries with amplified RNA
Method of defining cell types by probing comprehensive expression libraries with amplified RNA
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机译:通过使用扩增的RNA探索综合表达文库来定义细胞类型的方法
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摘要
The invention provides methods and compositions for defining a cell type, generally involving the steps of (a) amplifying the mRNA of a single cell of a heterogenous population of cells; (b) probing a comprehensive expression library with the amplified mRNA to define a gross expression profile of the cell; and (c) comparing the gross expression profile of the cell with a gross expression profile of one or more other cells to define a unique expression profile of the cell, wherein the unique expression profile of the cell provides a marker defining the cell type.
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