The in vitro assay of the cancerating substance which uses the phenotype transformation of the human cell

摘要

(57) Abstract Manner in order to evaluate the carcinogenesis of the chemical compound which uses the transformation assay is disclosed. Manner includes the fact that the chemical compound which is tested concerning carcinogenesis is made to contact the test cell. As for the test cell, it possesses the defect in the cell defense mechanism which is chosen from the group which consists of with defect and the defect in adjusting in cell cycle and in the capacity which prevents the damage with oxygen freely radical the defect in DNA damage restoration feature. You can see the feature of transformation, or not being seen the cell growth is evaluated in order to specify. The chemical compound which is tested due to the fact that feature of transformation occurs, having carcinogenesis is shown. The further example, the departure cancer/gun material which possesses systematic peculiarity manner in order to specify, the carcinogenic biochemical action mechanism of chemical compound includes the manner in order to specify, and the manner in order to evaluate the anti- carcinogenesis of chemical compound.