In vitro differentiation of vascular smooth muscle cells, methods and reagents related thereto

摘要

(57) [Abstract] The present invention relates to an in vitro system to induce rapid and uniform differentiation into vascular smooth muscle cells immortalized neural crest cells. If it is a phenotypic response for the development of peripheral arterial diseases, the in vitro system of the present invention, excessive proliferation of vascular smooth muscle cells, in order to identify the molecular regulators of growth and differentiation of smooth muscle cells it is used for. If the molecular regulators of smooth muscle cell differentiation are identified, the present invention also includes a method of isolating genes encoding these regulators. The present invention relates to molecules identified by the use of an in vitro system of the present invention relates to compounds that modulate or inhibit molecules and identified.