Compositions for measuring resistance and methods, and compositions for screening antiviral agents and methods and anti-viral drug susceptibility

摘要

(57) [Abstract] The present invention provides a method for determining the sensitivity of the HCMV antiviral agents or HCV: introducing into a host cell a resistance test vector comprising a marker gene and a segment derived from the patient (a) a step of culturing a host cell obtained step (b) in (a);; the steps of a step for determining the expression of the indicator gene in (c) target host cell, to give (d) in step (c) and a step of comparing the expression of the indicator gene measured when implemented steps (a) ~ (c) a in the absence of the antiviral agent, the expression of the indicator gene, steps (a) process - the process (c), the step (b) ~ process (c), or (c), the is to provide a method for causing the presence of HCV antiviral drug test concentration. The present invention provides a method for measuring the HCMV antiviral drug resistance or HCV in a patient: at the beginning (a), to measure the anti-viral drug susceptibility in the patient according to the above method, the segment from said patient The antiviral agent (c) steps (a) and it is measured in (b); at the time of (b) after the step of measuring the anti-viral drug susceptibility of the same patient; the step of obtaining from the patient at that time generally is reduction in time and a step of comparing the sensitivity and compared with sensitivity at the time of the first antiviral sensitivity at the time of the post, a way to indicate the development or progression of anti-viral drug resistance in the patient provide. The present invention also provides a method for evaluating the biological effects of HCMV antiviral drug candidate compounds or HCV. Resistance test vector comprising a patient-derived segment containing the HCMV gene or HCV, the indicator gene and host cells infected with said resistance test vector is provided.