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The head of the rice plant it is and has and can identify resistance indirectly the numerator marker

机译:水稻的头部是并且具有并且可以间接识别出抗性的分子标记

摘要

PROBLEM TO BE SOLVED: To obtain a molecular marker used when proliferating DNA fragment specific to rice blast resistance from a genome DNA extracted from rice individual and capable of judging presence of rice blast resistant gene by using specific oligonucleotides. ;SOLUTION: This molecular marker closely links to a rice blast-resistant gene represented by Pb-1(t) and identifies individual having rice blast resistance or sensibility. The molecular marker is used when DNA fragment specific to rice blast resistance is proliferated from a genome DNA extracted from rice individual in PCR reaction and the marker is a pair of two kinds of oligonucleotide (B1 primer) comprising base sequences of 5'-CCG GAA CAA TCT TCT GTC AC-3' and 5'-GCA GCT CAC TTT ATA TAA AGT-3'.;COPYRIGHT: (C)2000,JPO
机译:要解决的问题:要获得一种分子标记,当从水稻个体提取的基因组DNA中扩增出针对稻瘟病抗性的DNA片段时,该分子标记能够通过使用特定的寡核苷酸判断稻瘟病抗性基因的存在。 ;解决方案:该分子标记与Pb-1(t)代表的水稻抗稻瘟病基因紧密相关,可鉴定具有水稻抗稻瘟病或敏感性的个体。当在PCR反应中从水稻个体提取的基因组DNA中扩增出对稻瘟病抗性特异的DNA片段时,使用该分子标记,该标记是一对包含5'-CCG GAA碱基序列的两种寡核苷酸(B1引物)对CAA TCT TCT GTC AC-3'和5'-GCA GCT CAC TTT ATA TAA AGT-3'.;版权所有:(C)2000,JPO

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