Novel antitumor substance, microorganism and method for producing the substance, and cell cycle inhibitor and antitumor agent containing the substance as active ingredient

摘要

PROBLEM TO BE SOLVED: To obtain the subject new substance having animal cell specific proliferation inhibiting activity and cell circle inhibiting activity, by culturing a fungus belonging to the genus Aspergillus. SOLUTION: This substance has the following physical and chemical properties. Molecular weight: 350. Molecular formula: C20 H22 N22 O2 . Infrared absorption spectrum: as shown by the figure.1 H-Nuclear magnetic resonance: (ppm) 1.47(6H, s), 2.94(1H, dd, J=14, 10Hz), etc.13 C-Nuclear magnetic resonance: (ppm), 28.07(CH3 ), 28.07(CH3 ), etc.15 N-Nuclear magnetic resonance: (ppm), 112, 134, 161, 253. The maximum absorption value of ultraviolet light spectrum in methanol has a peak at 230nm under a neutral condition at 323nm. Being excited under a neutral condition in methanol by an ultraviolet light at 320-340nm, having the maximum value at 395-400nm and emitting fluorescence having 350-550nm wavelength width. Soluble in ethyl acetate, etc., slightly soluble in water, benzene, etc. Negative in ninhydrin reaction and positive in color reaction, in orange with nitrous acid. Color: white. Shown by the formula, produced by Aspergillus ustus (FERM P-15829).