DNA POLYMERASE B VARIANT EXHIBITING ENHANCED PERFORMANCE IN PCR

摘要

PROBLEM TO BE SOLVED: To provide a thermostable variant of a DNA polymerase B exhibiting an enhanced performance in PCR. ;SOLUTION: This thermostable DNA polymerase B variant suitable for polymerase chain reaction, is characterized by that amino acid(s), preferably tyrosine(s), is/are substituted in the Y-GG/A amino acid motif existing between N-terminal 3'-5'-exonuclease domain and C-terminal polymerase domain of wild-type DNA polymerase B.;COPYRIGHT: (C)2001,JPO