Is revealed at the time of flower bud formation of the tobacco the gene

摘要

PURPOSE:To obtain the title gene useful for culture of field crops, manifesting during formation of flower bud of tobacco by integrating a gene into a plant material to manifest the gene and controlling flowering by suppressing manifestation of the gene, using anti-sense RNA technology. CONSTITUTION:Pieces of floral axis epithelium of tobacco are cultured in a medium containing kinetin and indoleacetic acid, culture tissue is ground in a buffer solution, centrifuged, a supernatant liquid is recovered, put on a layer of 5.7M cesium chloride solution in a centrifugal pipe, ultra-centrifuged, precipitate is recovered, dissolved in a buffer solution, passed through an oligo alphaT cellulose column and an adsorbed substance is eluted to give mRNA. Then the mRNA is used as a template to prepare a cDNA library, which is screened with a probe, a positive clone is selected, a gene is recovered from the clone, treated with a restriction enzyme to give the objective gene encoding an amino acid sequence shown by the formula manifesting during the formation of flower bud of tobacco.