首页> 外国专利> CHIP FOR DETECTING POLYNUCLEOTIDE AND APPARATUS FOR TESTING POLYNUCLEOTIDE

CHIP FOR DETECTING POLYNUCLEOTIDE AND APPARATUS FOR TESTING POLYNUCLEOTIDE

机译:检测多核苷酸的芯片和测试多核苷酸的装置

摘要

PROBLEM TO BE SOLVED: To provide a chip for detecting a polynucleotide which allows binding/ forming complementary chains for each probe at an optimal temperature.;SOLUTION: Chip 100-1 for detecting a polynucleotide has sections 101-104 which are fixed for each of different oligonucleotide probes, a heating circuit formed for each section, and a temperature-detecting circuit formed for each of section, wherein the heating circuit and the temperature-detecting circuit of each section are formed so as to be operated independently for each section. This allows binding/forming complementary chains at a temperature of a Tm value of binding of complementary chain binding of each probe, so that there is substantially no difference in the formation amount of binding of complementary chains and the mismatch probability caused by a Tm difference.;COPYRIGHT: (C)2000,JPO
机译:解决的问题:提供一种用于检测多核苷酸的芯片,该芯片允许在最佳温度下结合/形成每个探针的互补链。解决方案:用于检测多核苷酸的芯片100-1具有针对每个探针固定的部分101-104。不同的寡核苷酸探针,为每个部分形成的加热电路和为每个部分形成的温度检测电路,其中每个部分的加热电路和温度检测电路形成为对于每个部分独立地操作。这允许在每个探针的互补链结合的结合的Tm值的温度下结合/形成互补链,使得互补链的结合的形成量和由Tm差异引起的错配概率基本上没有差异。 ;版权:(C)2000,日本特许厅

著录项

  • 公开/公告号JP2000342264A

    专利类型

  • 公开/公告日2000-12-12

    原文格式PDF

  • 申请/专利权人 HITACHI LTD;

    申请/专利号JP19990154655

  • 申请日1999-06-02

  • 分类号C12N15/09;C12M1/00;C12Q1/68;G01N33/483;G01N33/50;

  • 国家 JP

  • 入库时间 2022-08-22 01:28:20

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