首页> 外国专利> Isolated Nucleic Acid fragment that encodes an enzyme modifier of fatty acids of Vernonia galamenensis, Chimeric Gene, Cell huespedtransformada, transformed plant, SEED,Soybean, method for altering the level of expression of this Enzyme, method for producing acid

Isolated Nucleic Acid fragment that encodes an enzyme modifier of fatty acids of Vernonia galamenensis, Chimeric Gene, Cell huespedtransformada, transformed plant, SEED,Soybean, method for altering the level of expression of this Enzyme, method for producing acid

机译:分离的核酸片段,其编码加拉曼海藻,嵌合基因,huespedtransformada细胞,转化植物,SEED,大豆的脂肪酸的酶修饰剂,改变该酶表达水平的方法,产生酸的方法

摘要

Reveal isolated Fragments of nucleic acids which encode Plant enzymes involved in the biosynthesis and Modification of Fatty AcidsDondedichas Enzymes have Sequence Homology with the Membrane Fatty Acid desaturases of everyone.Specifically, isolated Fragments of u00e1cidosnucleicos and their complementary encoding an enzyme epoxidizante Enzyme of fatty acids and unsaturated fatty acids.Each of the first expressed in developing Seeds of Vernonia galamenensis.It also describes Chimeric genes comprising such Fragments of acids nucleicosligados operationally suitable Regulatory sequences.The expression of genes qiumu00e9ricos results in the production of altered levels of the Protein encoded in the host cells, which is also described.Also included are transformed plants obtained from huu00e9spedtransformadas cells of Higher Plants and seeds obtained from these transformed plants as well as a SEEDSoybean vernolico acid accumulates.In One embodiment, additional reveals a method for altering the expression levels of the Enzyme Fatty Acid epoxidizante Vernonia galamenensiso of Fatty Acid desaturase EnzymeVernonia galamenensis in a host cell. Yet Another embodiment comprises a method for producing u00e1cidosgrasos epoxidizados in Seeds of plants and a method for producing such Enzymes.It also provides a method to obtain Fragments of u00e1cidosnuclu00e9icos encoders all or virtually all of the amino acid sequences which encode Enzymes epoxidizantes or desaturases dAnd that grasosy acid products are obtained.
机译:揭示编码参与脂肪酸生物合成和修饰的植物酶的核酸的分离片段唐氏鱼类的酶具有与每个人的膜脂肪酸去饱和酶相同的序列同源性。首次在发育中的Vernonia galamenensis种子中表达。它还描述了包含此类核酸片段的嵌合基因,可操作地调节序列.qium u00e9ricos基因的表达导致蛋白质水平改变的产生。在一个实施方案中,还公开了一种从植物的hu.spedtransformadas细胞获得的转化植物和从这些转化植物获得的种子以及SEED大豆vernolico累计。交替蛋白脂肪酸脱氢酶酶加拉门氏菌(Vernonia galamenensis)的酶脂肪酸环氧化Vernonia galamenensiso在宿主细胞中的表达水平。另一个实施方案包括在植物种子中产生环氧化酶的方法和产生这种酶的方法。它还提供了一种获得编码环氧化物酶的全部或几乎所有氨基酸序列的编码器片段的方法。或去饱和酶d,就获得了草so酸产物。

著录项

  • 公开/公告号AR013928A1

    专利类型

  • 公开/公告日2001-01-31

    原文格式PDF

  • 申请/专利权人 E.I. DU PONT DE NEMOURS AND COMPANY;

    申请/专利号AR1998P102771

  • 发明设计人

    申请日1998-06-11

  • 分类号C12N9/00;A01H5/00;C12N1/00;C12N15/63;

  • 国家 AR

  • 入库时间 2022-08-22 01:26:20

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