Polypeptides comprising a portion of an immunogenic Antigen of M.Tuberculosis soluble, and DNA molecules encoding such polypeptides, expression vector Resistance comprising Said DNA Molecules, host cells transformed with these vectors.The pharmaceutical compositions.

摘要

Compounds and methods are presented for the Induction of Protective Immunity against tuberculosis.The supplied include polipu00e9ptidosque thereof comprise at least a portion of one or more immunogenic Proteins of M. Tuberculosis and DNA molecules encoding such polypeptides.The citadoscompuestos can be formulated in the form of Vaccines and / or Pharmaceutical compositions for Immunization against infection of M.Tuberculosis, or bienpueden be used for diagnosis of tuberculosis.As an example of these polypeptides may include the following: (a) ASP Pro Ala - ASP Val Val ile ASN THR THR CYS ASN Tyr Gly - Gln Val Val ala ala leu; (SEQ ID.HL 120) (b) - Val Glu ala Gly Leu met be - ala Gly THR Leu Pro Ala pro ser (SEQ ID no 121; (c) met ala ala Lys pro ARG Gly ASP THR Gly pro Leu Glu ala Lys Glu Gly ARG (SEQ ID ns122); (d) Tyr Tyr TRP Cys -Pro Gly GLN pro PHE Pro Ala - ASP TRP Gly Pro; (SEQ ID no. 123) (e) ASP ile Glu Gly ser THR ser Glu ASP XAA ala GLN Val (SEQ ID no 124. (f) ala Glu Glu ile THR - - - - be XAA XAA Glu ile Val Pro; (SEQ Id.Ns125) (g) - Pro Glu ASP Pro Ala pro Pro Val pro THR ala ser pro Pro ser (SEQ ID no: 126) (H) ala Lys - Pro - Thr XAA Tyr Glu Glu Leu - Thr Lys Gly - ASP THR Gly (SEQ ID ns127); (i) ASP Pro Ala pro ala - ASP - VThe Pro - Thr ala GLN THR Leu - Leu - Leu - - - - - - be ASN Leu ASP Pro Ala ASN Val PHE ala ASN ser; (SEQ ID id.seq. Hl (128).HL 136) (k) ASP Pro ASP Pro his GLN ASP Xaa - met THR Lys Gly Tyr Tyr pro Gly Gly ARG ARG PHE (SAA; id seq.129) and hl (L) - Tyr ile XAA XAA ala Tyr THR ala Gly ile Val pro Gly Lys ile ASN Val his Leu Val (SEQ ID.HL 137) where XAA can be any amino acid or where the Antigen comprises a sequence of aminou00e1cidoscodificada by a sequence of DNA that is selected from the group consisting ofS are listed in sequence SEQ ID.HL, 1, 4, 10, 13, 25, 52, 99 and 101Los bathroom these sequences, and the DNA sequences that hybridize to form a sequence in SEQ ID lists.HL - 1,2,4 - 10, 13 and 25, 3440, 101 or complement them in moderately severe conditions, comprising an Amino Acid Sequence codificad by a sequence of DNA that is