Viral expression system transgenico torque, method of revision of expressed gene in a plant or plant intrinsic transgene.Medoto of change of levels of a protein encoded by a gene target in a plant and system of replicacao viral.

摘要

"Transgenic viral expression system torque, method of alteration of the expression of endogenous plant gene or transgene in a plant.Method for modification of a proteu00ecna nu00ecveis encoded by a gene in a plant and target system of viral replication "this invention relates to a viral expression system in plants binu00e1rWas regulated. It includes two chromosomally integrated components.A component is a proreplicon, which contains sequ00b3u00eancias viral CIS action necessary for the replication and contains a gene target.The other component is a gene for replication of trans action which comprises a promoter regulated operationally connected to the region coding for a protein of viral replication.The proreplicon contains the gene of replication is essential for the replication of replicon, and thus can not submit to episomal autonomous replication.However, the expression regulated protein in the cells of replication of action trans plants also contains the proreplicon will trigger the release of free replicon proreplicon integrated.Results in its replication episomal in trans, and results in the expression of the target gene, if present, through amplification of genes.The expression system is useful for the production of foreign proteins, as well as the silencing of endogenous genes and transgenes in plant tissue.The tissue specific expression is controlled by the choice of the promoter controlling the transcription of the gene for replication of trans action.