The cDNA isolated, expression vector, host cell, starch synthase enzyme, antibody, polipeptu00ecdeo, transgenic plant, the process to produce starch, construction of fusion, proteu00ecna merger,Sequence of nucleotu00ecdeo Anti Sense DNA sequence, and starch.

摘要

The cDNA isolated, expression vector, host cell, starch synthase enzyme, antibody, polipeptu00ecdeo, transgenic plant, the process to produce starch, construction of fusion, proteu00ecna merger,Sequence of nucleotu00ecdeo Anti Sense DNA sequence, and starch. The gene of maize Dulli (DUI) of the present invention is a determinant of the structure of starch in the endosperm.The mutations of Dui affect the activity of at least two enzymes involved in the biosynthesis of starch or starch synthase, SSII, and branching enzyme of starch, SBEIIa.Dui encoding a predicted protein of 1674 residue, and is expressed as a temporal pattern unique in endosperm, but is not detectable in leaf or root.The size of the product DUI and its expression pattern marry precisely in the known characteristics of maize SSII.The product contains two different regions repeated Dui in its end amino unique, one of which is identical to a segment conserved enzymes desramificando starch.The cDNA encoding data provided by present invention, and mutations in this gene affects multiple aspects of biogu00eanese starch by the rupture of a complex of enzyme containing starch synthase (s),The enzyme (s) of starch branching enzyme (s), and possibly of desramificau00e7u00e3o starch.