Polypeptide of H.Medicinalis; Gene; production process; Pharmaceutical formulation that comprises; its use in the treatment of Thromboembolism; Antibody against the polypeptide and their use.Ex vivo Detection of Ra saratina; and Method for selecting inhibitors of platelet adhesion.

摘要

Describes a natural protein isolated from the saliva of the medicinal Leech Hirudo medicinalis, which strongly binds to Collagen.Thus acting as a natural inhibitor of the adhesion of platelets to Collagen Protein has a Molecular Weight of approximately 12000.An acidic isoelectric Point and contains Six cysteines. The Protein was sequenced, and the Gene was cloned from the DNA Library of H. medicinalis.Also disclosed are processes for producing the polypeptide by recombinant techniques.Both natural and Chimeric Proteins are potent inhibitors of the adhesion of platelets to Collagen dependent and thereforeCan be used for the therapeutic Treatment of various conditions related to Heart Disease and Diseases of the Circulatory System.In addition,The Protein is also suitable for natural or artificial Collagen coated surfaces to make them nonadhesive for cells and thereby prevent activSpecific cells.