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Immobilization of penicillin G amidase, glutaryl-7-ACA acylase or D-amino acid oxidase on an aminofunctional organosiloxane polymer carrier, use of the enzymes and process of their preparation
Immobilization of penicillin G amidase, glutaryl-7-ACA acylase or D-amino acid oxidase on an aminofunctional organosiloxane polymer carrier, use of the enzymes and process of their preparation
In the present invention there is described an enzyme being selected from a group comprising penicillin-G amidase /E.C.3.5.1,11/, glutaryl-7-ACA acylase and D-amino acid oxidase that is immobilized by covalent bonding on an aminofunctional organosiloxane polymer carrier. Further there are described individually the aforementioned three enzymes, as well as use of the enzymes being immobilized on a carrier during a corresponding enzymatic reaction, wherein the reaction temperature ranges between 4 to 30 degC and the reaction is carried out in the presence of an organic solvent. In the end there is also described a process for preparing enzymes immobilized on a carrier, which preparation process is characterized in that the enzyme is immobilized by covalent bonding on an aminofunctional organosiloxane polymer carrier.
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