Fremgangsmåte for fremstilling av dyrkning av celleaggregater referert til som primmorfer fra dissosierte celler fra svamper, koraller og andre invertebrater, samt anvendelse av disse

摘要

Marine ecol of sponges or coral, characterized in that it multicellular units are, of individual cells are associated under defined conditions and which are capable of, for several months to be held in a culture, wherein the primmorphs be obtained by:(1) Cutting of tissue samples from coral sponges or into smaller pieces of tissue,(2) Transferring the pieces of tissue into a volume of ca²⁺ and mg²⁺-free sea water, which contains ethylenediaminetetraacetic acid (edta),(3) and a gentle rotation of the volume of ca²⁺ and mg²⁺-free sea water, which contains the pieces of fabric, as a result of which cells is produced containing supernatant,(4) Filtration of the cell supernatant by a net and collection of the cells in suspension in a culture vessel,(5) Repeating step (4),(6) Combining the cell suspensions of step (5), in order to produce a combined cell suspension,(7) Incubating the cell suspension in sea water / antibiotic - solution,(8) Adjusting the cell suspension usually 10 to⁷ Cells / ml,(9), and are usually transferring..