Electrochemical probes for detection of molecular interactions and drug discovery

摘要

A method and apparatus for performing potentiometric analyses for detecting specific binding between a first member of a biological binding pair immobilized on an electrode and a second member of a biological binding pair that is electrochemically labelled, in the presence of an electrochemical mediator. The second member comprises a peptide electrochemically labelled with a transition metal complex. The presence or absence of binding of the labelled second member is detected by the presence or absence of a current created by applying a voltage to the electrodes to generate oxidation/reduction reactions. Methods for using the apparatus for performing binding and competition binding assays are provided as well as methods for performing high throughput screening assays for detecting inhibition of specific binding between the members of the biological binding pair for use in drug development, biochemical analysis and protein purification assays.