PROCESS FOR PREPARING AN ACTIVE PRODUCT FOR REGULATING INTESTINAL FLORA IN HUMANS

摘要

The invention relates to a process for preparing an active product regulating the intestinal flora in humans by biosynthesis, by means of the microorganism Bacillus subtilis, said process comprising the following stages: the inoculum production by growing the strain I.C.C.F.-77A in a medium containing corn extract and mineral salts, at 30...32°C for 18...20 hours, the bioprocess as such by vegetative growth in an identical medium and under the same conditions, with maximal sporulation of the bacterial population in a ratio of more than 90% for 22...26 hours, settling and centrifuging at 3,000 rpm for 20...30 min while cooling, drying the sporulated biomass by lyophilization, finally producing a powder with 1.7x10 10...2x10 12 spores/gramme, raw protein with 8...15% dry substance and free amino acids: aspartic acid, valine, leucine, gluconic acid, alpha-alanine and heavy metals 0.001%.