Integration vector of an independent site gene expression in mammalian host cells.

摘要

A TABLE FOR INTEGRATION IN A GENE GENETIC MATERIAL OF A mammalian host cell such that the gene can be expressed by the host cell. TABLE CONSISTS OF A PROMOTER GENE AND REGION CONTROL immune globulin DOMINANT DERIVATIVE OF LOCUS OF GENE immune globulin mouse CAPABLE LAMBDA removing a host cell restrictor type, a position of INTEGRACION INDEPENDENT copy number DEPENDENT EXPRESSION that gene. POSITIONS SUPER hypersensitive DNase exemplified ARE I) NEAR 2.35 KB above the CAP POSITION GEN LAMBDA, REORDERED II) About 2.5 KB ABOVE SEGMENT genomic vlambda SUB 2 O III) About 30 KB DOWN THE GENE LAMBDA, ADAPTED. THE mammalian host cells transformed with the vector ARE REVEALED AS TRANSGENIC MAMMALS ARE PROCESSED WITH TABLE AND A METHOD OF PRODUCING A POLYPEPTIDE INCLUDING A culture of transformed mammalian cell. A method of therapy GENETICS includes the steps of I) kill cells TRUNK mammalian body, II) optionally KILL THE REST OF THE TRUNK body cells, III) TRANSFORMATION OF CELLS TRUNK LEARNED WITH VECTOR CONTAINING A POORLY GEN O ABSENT IN THE BODY, AND IV) also revealed REPLACING THE STEM CELLS TRANSFORMED IN THE BODY. ALSO REVEALED THE FUNCTIONAL ESTIMULADOR Immunoglobulin LAMBDA, mouse, consisting of a DNA sequence consisting of all or part FUNCTIONAL DNA sequence BETWEEN EcoRI site 3.8 KB below the XhoI POSITION GEN REORDERED and position SnaBI 10KB BELOW THIS POSITION XhoI. FUNCTIONAL ESTIMULADOR mouse immunoglobulin MAY CONTAIN ALL OR PART I) THE FIRST TO 1.3KB HINDIII DNA fragment HindII DOWN POSITION 3.8 KB CEN DOWN POSITION IN GEN REORDERED XhoI mouse, II ) 3.3 KB HINDIII TO HINDIII DNA fragment DOWN POSITION 3.8 KB CEN DOWN POSITION IN GEN XhoI LAMBDA, REORDERED mOUSE AND REACHING THE POSITION SnaBI 10KB down this position XhoI.