Chimeric genes using cDNA in antisense orientation of the glucose-2 transporter (GLUT2) and transgenic non-human animals expressing said chimeric genes for use in the study of diabetes mellitus and the development of therapeutic approaches to the disease.

摘要

Chimeric genes using cDNA of the glucose-2 transporter (GLUT2) in antisense orientation and transgenic non-human animals expressing said chimeric genes for use in the study of diabetes mellitus and the development of therapeutic approaches to the disease.Concerns a chimeric gene using cDNA (complementary DNA) of the glucose-2 transporter (GLUT2) in reversed orientation, directed by a promoter or promoter fusion.It also concerns a chimeric gene obtained by the fusion of GLUT2 cDNA in antisense orientation with the insulin gene promoter and a chimeric gene obtained by fusion of GLUT2 cDNA in antisense orientation with the P-enolpyruvate carboxykinase promoter (PEPCK).It also concerns an expression vector for the expression of antisense GLUT2 RNA in any cell type, notably a pRIPantiGT2 and pInsantiGT2 plasmid vector, a pPCKantiGT2 plasmid vector, a vRIPantiGT2 and vInsantiGT2 retroviral vector and a pPCKantiGT2 retroviral vector.It also concerns transgenic non-human animals which express the aforementioned chimeric gene and a cell type originating in said transgenic non-human animals.Applies to the development of therapeutic approaches to diabetes mellitus.