automatic assignment lineage of acute leukemia by flow cytometry.

摘要

It PRESENTS A METHOD FOR AUTOMATED GENEALOGY SSIGNMENT leukemias. EIGHT IS ACQUIRED DATA FILES MODE list of four parameters with a flow cytometer in the following sequence: 1. CLEAN; 2. CONTROLS ISOTOPES; 3. CD10 FITC, CD19 PE; 4. CD20 FITC, PE CD5; 5. CD3 FITC, CD22 PE; 6. CD7 FITC, CD33 PE; 7. HLADR FITC, CD13 PE CD34 FITC 8. And, CD38 PE. FIRST DATA FILES 3-8 GROUPED using an algorithm based on VICINITY OF OLDER. GROUPS JOIN TOGETHER THEN ACROSS THE DATA FILES FOR FORMING CELL POPULATIONS USING THE ASSUMPTION OF invariance of the light scattering through tubes for a certain location. POSITIONS means of each cell population compared to a decision tree IDENTIFY POPULATIONS normal cells. TO IDENTIFY leukemic cell populations, the algorithm ELIMINATES cell populations NORMAL SPACE DATA AND REMAINING STOCKS ARE CLASSIFIED AS ALL GENEALOGIA B, GENEALOGIA T ALL, AML, AUL, B-CELL or unknown.