METHOD FOR DETECTION OR DETERMINATION OF HCV CORE ANTIGENS AND REAGENT FOR DETECTION OR DETERMINATION FOR USE THEREIN

摘要

The present invention provides an immunoassay for thedetection of HCV core antigens in a sample which comprisessubjecting an alkaline phosphatase-labeled anti-HCV coreprotein antibody and an HCV core antigen in the sample toantigen-antibody reaction in an aqueous solution containing asalt at a high concentration to form an antigen-antibody complex,and measuring the alkaline phosphatase activity of the formedantigen-antibody complex. Also provided is a reagent for thedetection of HCV core antigens comprising an alkalinephosphatase-labeled anti-HCV core protein antibody, a salt ata concentration of 0.5 M to saturation and a reagent for themeasurement of alkaline phosphatase activity.