A METHOD FOR TYPING POLYMORPHISMS OF BOVINE MHC CLASS II GENES

摘要

A primer set used in PCR for accurately and rapidly typingpolymorphisms of DNA encoding BoLA-DRB3.2 which comprises:(1) a reverse primer capable of amplifying all alleles of BoLA-DRB3.2; and(2) a forward primer capable of amplifying all alleles in any one of two ormore groupsof alleles of BoLA-DRB3.2 wherein each of said groups comprises at least oneallele,but incapable of amplifying allele(s) contained in the other group(s).