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Quantification of cellular injury using expression of a fluorescent protein under the control of the GADD153 promoter
Quantification of cellular injury using expression of a fluorescent protein under the control of the GADD153 promoter
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机译:使用GADD153启动子控制下的荧光蛋白表达定量细胞损伤
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摘要
The present invention features a novel cellular injury reporter system in which a chimeric gene containing the GADD153 promoter linked to the coding region of an enhanced green fluorescent protein (EGFP) gene was stably integrated into the genome of carcinoma cells. Activation of the GADD153 promoter was quantified using flow cytometric measurement of EGFP expression following drug exposure. This reporter system is suitable for high. throughput in vitro and in vivo screening for agents capable of producing cytotoxicity via a wide variety of different mechanisms, and can be utilized to investigate the relative potency of structurally related DNA adducts.
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