ISOLATION OF NOVEL HEMAPOIETIC GENES BY REPRESENTATIONAL DIFFERENCE ANALYSIS

摘要

The invention encompasses a screening method for discovering novel erythrocytic genes, by PCR-based analysis of representational differences between two species of Antarctic fish, a red-blooded species and a white-blooded, hemoglobin- and erythrocyte-lacking species. The invention also encompasses methods of using PCR-based representational difference analysis to discover myelopoiesis and lymphopoiesis related genes from icefish of the Channicthyidae family of the Notothenioidei suborder. Also disclosed are new clones containing putative genes involved in erythropoiesis.