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BIOCONVERSIONS CATALYZED BY THE TOLUENE MONOOXYGENASE OF $i(PSEUDOMONAS MENDOCINA) KR-1
BIOCONVERSIONS CATALYZED BY THE TOLUENE MONOOXYGENASE OF $i(PSEUDOMONAS MENDOCINA) KR-1
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机译:$ i(伪单胞菌)KR-1的甲苯单加氧酶催化的生物转化
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摘要
Disclosed and claimed are toluene monooxygenase (TMO) gene sequences from Pseudomonas mendocina KR-1, TMO proteins encoded by these sequences, recombinant plasmids containing such sequences, and microorganism host cells containing such plasmids. A five-gene and six-gene TMO gene cluster encode proteins that are useful in a variety of bioconversions. In particular, the TMO gene cluster is useful for the preparation of p-hydroxyphenylacetic acid and indigo. In addition, the TMO gene cluster is useful for the degradative bioconversion of toxic compounds, such as trichloroethylene.
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