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A METHOD FOR THE SELECTION AND GROWTH OF OOCYTES SUITABLE FOR EMBRYONIC DEVELOPMENT

机译:一种适合胚胎发育的卵母细胞的选择和生长的方法

摘要

The present invention concerns a method for the culture of oocytes suitable for embryonic development in a synthetic medium containing taurine. The oocytes, coming from female mammals, are pre-selected SN oocytes based on the morphology of the nuclear chromatin, highlighted with Hoechst 33342 fluorochrome dye, matured and fertilized in vitro, and then grown, until the blastocyst stage, in a synthetic medium containing taurine.
机译:本发明涉及一种在含有牛磺酸的合成培养基中培养适于胚胎发育的卵母细胞的方法。来自雌性哺乳动物的卵母细胞是根据核染色质的形态预先选择的SN卵母细胞,用Hoechst 33342荧光染料突出显示,在体外成熟并受精,然后在含有以下成分的合成培养基中生长直至胚泡阶段:牛磺酸。

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