- / PURIFICATION AND/OR CONCENTRATION OF DNA BY CROSS-FLOW FILTRATION SEPARATION OF ENDOTOXINS FROM A NUCLEIC ACID PREPARATION

摘要

The present invention while in contact with a solution containing the nucleic acid in the semipermeable membrane is passed through once or several times, the nucleic acid molecules are retained by the membrane, the more material that has a lower molecular weight is purified and / In can be adsorbed to the membrane to pass through and / or membranes or to obtain a concentrated nucleic acid solution, a solution relates to a method for purifying or / and concentration of nucleic acid in the. The same procedure is carried out to separate endotoxins from a nucleic acid preparation. Additionally, the present invention is a nucleic acid cross for the purification and / or concentration and separation of endotoxin from nucleic acid in the solution preparation of the - and the use of the flow filtration system. The present invention also cross-cloning of the nucleic acids purified and / or concentrated by flow filtration, transformation, Tran specification design, microinjection into the cell, the use in gene therapy, DNA vaccination and / or a polymerase chain reaction It relates to the use for the (PCR).