-1 NOVEL MODIFIED MSP-1 NUCLEIC ACID SEQUENCES AND METHODS FOR INCREASING mRNA LEVELS AND PROTEIN EXPRESSION IN CELL SYSTEMS

摘要

The present invention relates to modified recombinant nucleic acid sequences (preferably DNA) and methods for increasing mRNA levels and protein expression of malaria surface protein MSP-1, which are known to be difficult to express in cell culture systems, mammalian cell culture systems or mutant animals. will be. Preferred protein candidates for expression using recombinant techniques of the present invention are expressed from DNA encoding sequences with reduced total AT content or AT rich regions and / or mRNA instability motifs and / or rare codons as compared to native MSP-1 genes. MSP-1 protein.