Mutant of Phaffia rhodozyma producing astaxanthin and fermentation method thereof

摘要

PURPOSE: Provided is a method for separating mutant strain, Phaffia rhodozyma (KCTC-0920B) which generates astaxanthin in a high yield in a short period of time without being degenerated into a wild type during continuous cultivations. CONSTITUTION: The method for separating mutant strain, Phaffia rhodozyma (KCTC-0920B) comprises the steps of: i) cultivating Phaffia rhodozyma (ATCC-96594) in YM broth and treating the strain with 1-methyl-3-nitro-1-nitrosoguanidine (NTG) to kill 98% of yeast; ii) spreading the remaining yeast on YM medium for cultivation and selecting colonies which are growing faster with red color than the original strain; iii) repeating these processes several times and then, cultivating selected bacteria in YM broth for 4-6 days; and iv) measuring the amount of mycobiont and carotenoid and selecting the strain which produces carotenoid in a high yield.